Transcript: Mouse NM_145459.3

Mus musculus zinc finger protein 503 (Zfp503), mRNA.

Source:
NCBI, updated 2017-05-14
Taxon:
Mus musculus (mouse)
Gene:
Zfp503 (218820)
Length:
4251
CDS:
1329..3287

Additional Resources:

NCBI RefSeq record:
NM_145459.3
NBCI Gene record:
Zfp503 (218820)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_145459.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000340461 ATCGGGATACCCGCTAGTATA pLKO_005 2735 CDS 100% 13.200 18.480 N Zfp503 n/a
2 TRCN0000340460 CTCCAAACCCGGTTCAGATAA pLKO_005 1877 CDS 100% 13.200 18.480 N Zfp503 n/a
3 TRCN0000071945 CAAACCCGGTTCAGATAAGAA pLKO.1 1880 CDS 100% 5.625 7.875 N Zfp503 n/a
4 TRCN0000071943 CGGAATTAACGTGGACGTGAA pLKO.1 2210 CDS 100% 4.050 5.670 N Zfp503 n/a
5 TRCN0000141354 CCTAAGAAGCAGTAAGCACAG pLKO.1 1355 CDS 100% 2.250 3.150 N ZNF503 n/a
6 TRCN0000071944 CTACGGTTTCATGCTCCCTAA pLKO.1 2852 CDS 100% 4.050 3.240 N Zfp503 n/a
7 TRCN0000340459 GCCGGTGAACATTGGTATTTA pLKO_005 3589 3UTR 100% 15.000 10.500 N Zfp503 n/a
8 TRCN0000340539 CTCAAACATGTTCGCAGATAG pLKO_005 1693 CDS 100% 10.800 7.560 N Zfp503 n/a
9 TRCN0000351062 GCAACTGGGTGTCTGCTAATG pLKO_005 2893 CDS 100% 10.800 7.560 N Zfp503 n/a
10 TRCN0000071947 GACGACAAGAAAGATCCGGAA pLKO.1 2097 CDS 100% 2.160 1.512 N Zfp503 n/a
11 TRCN0000071946 CATGGCTTGTCACATGCACAT pLKO.1 3050 CDS 100% 4.050 2.430 N Zfp503 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_145459.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09227 pDONR223 100% 88.9% 95.5% None (many diffs) n/a
2 ccsbBroad304_09227 pLX_304 0% 88.9% 95.5% V5 (many diffs) n/a
3 ccsbBroadEn_16045 pDONR223 0% 84% 90.9% None (many diffs) n/a
4 ccsbBroad304_16045 pLX_304 0% 84% 90.9% V5 (many diffs) n/a
5 TRCN0000471349 CTGCCCAGGTGGACGCTTCCCGTC pLX_317 20.9% 83.9% 90.8% V5 (many diffs) n/a
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