Construct: shRNA TRCN0000072205
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- rfp_59s1c1
- DNA Barcode:
- GCTCCGTGAACGGCCACGAGT
Original Target:
- Taxon:
-
Control
- Gene:
- RFP (RFP)
- Gene Description:
- RFP
- Transcript:
- Broad rfp.1 (CURRENT)
- Match location:
- Position 59 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000072205)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTCCGTGAACGGCCACGAGT
- Hairpin Sequence:
-
5'-CCGG-GCTCCGTGAACGGCCACGAGT-CTCGAG-ACTCGTGGCCGTTCACGGAGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTCCGTGAACGGCCACGAGTCTCGAGACTCGTGGCCGTTCACGGAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTCCGTGAACGGCCACGAGTCTCGAGACTCGTGGCCGTTCACGGAGC-3'
Other clones with same target sequence:
-
TRCN0000231703
