Construct: shRNA TRCN0000072231

Construct Description:

Construct Type:
shRNA
Other Identifiers:
lacZ_1650s1c1
DNA Barcode:
CGCTAAATACTGGCAGGCGTT

Original Target:

Taxon:
Control
Gene:
lacZ (lacZ)
Gene Description:
lacZ
Transcript:
Broad lacZ.1 (CURRENT)
Match location:
Position 1650 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072231)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTAAATACTGGCAGGCGTT
Hairpin Sequence:
5'-CCGG-CGCTAAATACTGGCAGGCGTT-CTCGAG-AACGCCTGCCAGTATTTAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTAAATACTGGCAGGCGTTCTCGAGAACGCCTGCCAGTATTTAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTAAATACTGGCAGGCGTTCTCGAGAACGCCTGCCAGTATTTAGCG-3'

Other clones with same target sequence:

  1. TRCN0000231710