Construct: shRNA TRCN0000075301

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181533.3-591s1c1
DNA Barcode:
GCCCAGTTGATGCTATTGTCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ABHD12B (145447)
Gene Description:
abhydrolase domain containing 12B
Transcript:
RefSeq NM_181533.3 (CURRENT)
Match location:
Position 591 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000075301)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAGTTGATGCTATTGTCT
Hairpin Sequence:
5'-CCGG-GCCCAGTTGATGCTATTGTCT-CTCGAG-AGACAATAGCATCAACTGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAGTTGATGCTATTGTCTCTCGAGAGACAATAGCATCAACTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAGTTGATGCTATTGTCTCTCGAGAGACAATAGCATCAACTGGGC-3'

Other clones with same target sequence:

(none)