Construct: shRNA TRCN0000075669

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053225.1-515s1c1
DNA Barcode:
GTTCAATTCTACCTGTGAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vmn1r50 (113852)
Gene Description:
vomeronasal 1 receptor 50
Transcript:
RefSeq NM_053225.1 (CURRENT)
Match location:
Position 515 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000075669)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCAATTCTACCTGTGAGTT
Hairpin Sequence:
5'-CCGG-GTTCAATTCTACCTGTGAGTT-CTCGAG-AACTCACAGGTAGAATTGAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCAATTCTACCTGTGAGTTCTCGAGAACTCACAGGTAGAATTGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCAATTCTACCTGTGAGTTCTCGAGAACTCACAGGTAGAATTGAAC-3'

Other clones with same target sequence:

(none)