Construct: shRNA TRCN0000076333

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019911.2-1416s1c1
DNA Barcode:
GTGAGCGATTAGGAGGATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tdo2 (56720)
Gene Description:
tryptophan 2,3-dioxygenase
Transcript:
RefSeq NM_019911.2 (CURRENT)
Match location:
Position 1416 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000076333)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGAGCGATTAGGAGGATTAA
Hairpin Sequence:
5'-CCGG-GTGAGCGATTAGGAGGATTAA-CTCGAG-TTAATCCTCCTAATCGCTCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGAGCGATTAGGAGGATTAACTCGAGTTAATCCTCCTAATCGCTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGAGCGATTAGGAGGATTAACTCGAGTTAATCCTCCTAATCGCTCAC-3'

Other clones with same target sequence:

(none)