Construct: shRNA TRCN0000080367

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_052994.2-1063s1c1
DNA Barcode:
CTTCAACTCCTGTGATACCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spock2 (94214)
Gene Description:
sparc/osteonectin, cwcv and kazal-like domains proteoglycan 2
Transcript:
RefSeq NM_052994.2 (CURRENT)
Match location:
Position 1063 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000080367)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCAACTCCTGTGATACCTA
Hairpin Sequence:
5'-CCGG-CTTCAACTCCTGTGATACCTA-CTCGAG-TAGGTATCACAGGAGTTGAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCAACTCCTGTGATACCTACTCGAGTAGGTATCACAGGAGTTGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCAACTCCTGTGATACCTACTCGAGTAGGTATCACAGGAGTTGAAG-3'

Other clones with same target sequence:

(none)