Construct: shRNA TRCN0000080805

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025272.2-198s1c1
DNA Barcode:
TGTTGGTGACTTGTTCAGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp6v0e (11974)
Gene Description:
ATPase, H+ transporting, lysosomal V0 subunit E
Transcript:
RefSeq NM_025272.2 (CURRENT)
Match location:
Position 198 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000080805)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTTGGTGACTTGTTCAGTTT
Hairpin Sequence:
5'-CCGG-TGTTGGTGACTTGTTCAGTTT-CTCGAG-AAACTGAACAAGTCACCAACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTTGGTGACTTGTTCAGTTTCTCGAGAAACTGAACAAGTCACCAACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTTGGTGACTTGTTCAGTTTCTCGAGAAACTGAACAAGTCACCAACA-3'

Other clones with same target sequence:

(none)