Transcript: Mouse NM_025272.2

Mus musculus ATPase, H+ transporting, lysosomal V0 subunit E (Atp6v0e), mRNA.

Source:
NCBI, updated 2017-05-14
Taxon:
Mus musculus (mouse)
Gene:
Atp6v0e (11974)
Length:
800
CDS:
80..325

Additional Resources:

NCBI RefSeq record:
NM_025272.2
NBCI Gene record:
Atp6v0e (11974)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_025272.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000307600 TGATCGTGATGAGCGTGTTCT pLKO_005 108 CDS 100% 4.950 6.930 N Atp6v0e n/a
2 TRCN0000080803 CGGGAAATGTGTTACTCTTAT pLKO.1 553 3UTR 100% 13.200 10.560 N Atp6v0e n/a
3 TRCN0000288428 CGGGAAATGTGTTACTCTTAT pLKO_005 553 3UTR 100% 13.200 10.560 N Atp6v0e n/a
4 TRCN0000080807 TCCTCTGTTTGGACCACAGTT pLKO.1 262 CDS 100% 4.950 3.465 N Atp6v0e n/a
5 TRCN0000080804 TGAAACCATCTGGTATCTGAA pLKO.1 289 CDS 100% 4.950 3.465 N Atp6v0e n/a
6 TRCN0000080805 TGTTGGTGACTTGTTCAGTTT pLKO.1 198 CDS 100% 4.950 3.465 N Atp6v0e n/a
7 TRCN0000218687 TTCAGTTTGCTGCTATCTCTT pLKO_005 211 CDS 100% 4.950 3.465 N ATP6V0E1 n/a
8 TRCN0000307601 TTCAGTTTGCTGCTATCTCTT pLKO_005 211 CDS 100% 4.950 3.465 N Atp6v0e n/a
9 TRCN0000080806 GCTGATTGCAATTCTGGCACA pLKO.1 235 CDS 100% 2.160 1.512 N Atp6v0e n/a
10 TRCN0000295639 TACCACTGGCCTTGAGGATGA pLKO_005 311 CDS 100% 0.000 0.000 N Atp6v0e n/a
11 TRCN0000295640 GGGAGTTATCATCACCATGTT pLKO_005 181 CDS 100% 4.950 2.970 N Atp6v0e n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_025272.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02061 pDONR223 100% 90.5% 98.7% None (many diffs) n/a
2 ccsbBroad304_02061 pLX_304 0% 90.5% 98.7% V5 (many diffs) n/a
3 TRCN0000473095 CATCTCTTCATCAAACAACCATTC pLX_317 100% 90.5% 98.7% V5 (many diffs) n/a
Download CSV