Construct: shRNA TRCN0000081139
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_175935.3-860s1c1
- DNA Barcode:
- CCTCATGTATTGGACTCTCTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- G6pc3 (68401)
- Gene Description:
- glucose 6 phosphatase, catalytic, 3
- Transcript:
- RefSeq NM_175935.3 (CURRENT)
- Match location:
- Position 860 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000081139)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCTCATGTATTGGACTCTCTT
- Hairpin Sequence:
-
5'-CCGG-CCTCATGTATTGGACTCTCTT-CTCGAG-AAGAGAGTCCAATACATGAGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCTCATGTATTGGACTCTCTTCTCGAGAAGAGAGTCCAATACATGAGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCTCATGTATTGGACTCTCTTCTCGAGAAGAGAGTCCAATACATGAGG-3'
Other clones with same target sequence:
-
TRCN0000327548
