Construct: shRNA TRCN0000083123

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138702.1-736s1c1
DNA Barcode:
CCCGACCATTTCTATGTCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAGEC3 (139081)
Gene Description:
MAGE family member C3
Transcript:
RefSeq NM_138702.1 (CURRENT)
Match location:
Position 736 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000083123)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGACCATTTCTATGTCTTT
Hairpin Sequence:
5'-CCGG-CCCGACCATTTCTATGTCTTT-CTCGAG-AAAGACATAGAAATGGTCGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGACCATTTCTATGTCTTTCTCGAGAAAGACATAGAAATGGTCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGACCATTTCTATGTCTTTCTCGAGAAAGACATAGAAATGGTCGGG-3'

Other clones with same target sequence:

(none)