Construct: shRNA TRCN0000085012

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025850.2-920s1c1
DNA Barcode:
GCTGTCTTAAATAATCACGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fank1 (66930)
Gene Description:
fibronectin type 3 and ankyrin repeat domains 1
Transcript:
RefSeq NM_025850.2 (CURRENT)
Match location:
Position 920 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000085012)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTCTTAAATAATCACGAA
Hairpin Sequence:
5'-CCGG-GCTGTCTTAAATAATCACGAA-CTCGAG-TTCGTGATTATTTAAGACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTCTTAAATAATCACGAACTCGAGTTCGTGATTATTTAAGACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTCTTAAATAATCACGAACTCGAGTTCGTGATTATTTAAGACAGC-3'

Other clones with same target sequence:

(none)