Transcript: Mouse NM_025850.2

Mus musculus fibronectin type 3 and ankyrin repeat domains 1 (Fank1), mRNA.

Source:
NCBI, updated 2017-05-13
Taxon:
Mus musculus (mouse)
Gene:
Fank1 (66930)
Length:
2070
CDS:
68..1102

Additional Resources:

NCBI RefSeq record:
NM_025850.2
NBCI Gene record:
Fank1 (66930)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_025850.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000413861 ACTCATCACAGCATCGAATTG pLKO_005 128 CDS 100% 10.800 15.120 N Fank1 n/a
2 TRCN0000085009 GCCCATAAGTAGCGAACACTT pLKO.1 388 CDS 100% 4.950 6.930 N Fank1 n/a
3 TRCN0000085010 CGGTGTCATTTACACGGGTTA pLKO.1 244 CDS 100% 4.050 5.670 N Fank1 n/a
4 TRCN0000424138 CACCTAGATGTTGTCAAATAC pLKO_005 632 CDS 100% 13.200 9.240 N Fank1 n/a
5 TRCN0000085011 CCTAGAACCAAGGACACTATA pLKO.1 289 CDS 100% 13.200 9.240 N Fank1 n/a
6 TRCN0000416023 ACGTGAACATCAAGGACAAAG pLKO_005 876 CDS 100% 10.800 7.560 N Fank1 n/a
7 TRCN0000085008 CCCACATTGAAATACATCTTT pLKO.1 1253 3UTR 100% 5.625 3.938 N Fank1 n/a
8 TRCN0000085012 GCTGTCTTAAATAATCACGAA pLKO.1 920 CDS 100% 2.640 1.848 N Fank1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_025850.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09342 pDONR223 100% 85.7% 88.1% None (many diffs) n/a
2 ccsbBroad304_09342 pLX_304 0% 85.7% 88.1% V5 (many diffs) n/a
3 TRCN0000479772 AGGCAATCGTACGATGCCATACCT pLX_317 34.4% 85.7% 88.1% V5 (many diffs) n/a
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