Construct: shRNA TRCN0000086254

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172403.2-903s1c1
DNA Barcode:
GCTCGACGAATGCCTTTGATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2810021J22Rik (69944)
Gene Description:
RIKEN cDNA 2810021J22 gene
Transcript:
RefSeq NM_172403.2 (CURRENT)
Match location:
Position 903 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000086254)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCGACGAATGCCTTTGATA
Hairpin Sequence:
5'-CCGG-GCTCGACGAATGCCTTTGATA-CTCGAG-TATCAAAGGCATTCGTCGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCGACGAATGCCTTTGATACTCGAGTATCAAAGGCATTCGTCGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCGACGAATGCCTTTGATACTCGAGTATCAAAGGCATTCGTCGAGC-3'

Other clones with same target sequence:

(none)