Construct: shRNA TRCN0000087773

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_194060.1-2150s1c1
DNA Barcode:
CACCAATCTTAGTCATCCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Foxo6 (329934)
Gene Description:
forkhead box O6
Transcript:
RefSeq NM_194060.1 (CURRENT)
Match location:
Position 2150 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087773)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCAATCTTAGTCATCCATT
Hairpin Sequence:
5'-CCGG-CACCAATCTTAGTCATCCATT-CTCGAG-AATGGATGACTAAGATTGGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCAATCTTAGTCATCCATTCTCGAGAATGGATGACTAAGATTGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCAATCTTAGTCATCCATTCTCGAGAATGGATGACTAAGATTGGTG-3'

Other clones with same target sequence:

(none)