Construct: shRNA TRCN0000088669

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144909.1-1050s1c1
DNA Barcode:
CCTCGGTATCATCGCCATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gckr (231103)
Gene Description:
glucokinase regulatory protein
Transcript:
RefSeq NM_144909.1 (CURRENT)
Match location:
Position 1050 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000088669)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCGGTATCATCGCCATTAT
Hairpin Sequence:
5'-CCGG-CCTCGGTATCATCGCCATTAT-CTCGAG-ATAATGGCGATGATACCGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCGGTATCATCGCCATTATCTCGAGATAATGGCGATGATACCGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCGGTATCATCGCCATTATCTCGAGATAATGGCGATGATACCGAGG-3'

Other clones with same target sequence:

(none)