Construct: shRNA TRCN0000088670

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144909.1-679s1c1
DNA Barcode:
GCCAGAAATGATCCCATTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gckr (231103)
Gene Description:
glucokinase regulatory protein
Transcript:
RefSeq NM_144909.1 (CURRENT)
Match location:
Position 679 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000088670)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGAAATGATCCCATTGAA
Hairpin Sequence:
5'-CCGG-GCCAGAAATGATCCCATTGAA-CTCGAG-TTCAATGGGATCATTTCTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGAAATGATCCCATTGAACTCGAGTTCAATGGGATCATTTCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGAAATGATCCCATTGAACTCGAGTTCAATGGGATCATTTCTGGC-3'

Other clones with same target sequence:

(none)