Construct: shRNA TRCN0000088923

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009520.2-2685s1c1
DNA Barcode:
CCCAGCTATTTACTCTCTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wnt2b (22414)
Gene Description:
wingless-type MMTV integration site family, member 2B
Transcript:
RefSeq NM_009520.2 (NON-CURRENT)
Match location:
Position 2685 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000088923)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGCTATTTACTCTCTATT
Hairpin Sequence:
5'-CCGG-CCCAGCTATTTACTCTCTATT-CTCGAG-AATAGAGAGTAAATAGCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGCTATTTACTCTCTATTCTCGAGAATAGAGAGTAAATAGCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGCTATTTACTCTCTATTCTCGAGAATAGAGAGTAAATAGCTGGG-3'

Other clones with same target sequence:

(none)