Construct: shRNA TRCN0000090853

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008449.2-3930s1c1
DNA Barcode:
CCCAGTTGTTTGAACATCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kif5c (16574)
Gene Description:
kinesin family member 5C
Transcript:
RefSeq NM_008449.2 (CURRENT)
Match location:
Position 3930 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090853)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGTTGTTTGAACATCATT
Hairpin Sequence:
5'-CCGG-CCCAGTTGTTTGAACATCATT-CTCGAG-AATGATGTTCAAACAACTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGTTGTTTGAACATCATTCTCGAGAATGATGTTCAAACAACTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGTTGTTTGAACATCATTCTCGAGAATGATGTTCAAACAACTGGG-3'

Other clones with same target sequence:

  1. TRCN0000325140