Construct: shRNA TRCN0000090863

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023279.2-1605s1c1
DNA Barcode:
CCTCTGTATTTATGTTGCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tubb3 (22152)
Gene Description:
tubulin, beta 3 class III
Transcript:
RefSeq NM_023279.2 (CURRENT)
Match location:
Position 1605 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090863)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTGTATTTATGTTGCTTA
Hairpin Sequence:
5'-CCGG-CCTCTGTATTTATGTTGCTTA-CTCGAG-TAAGCAACATAAATACAGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTGTATTTATGTTGCTTACTCGAGTAAGCAACATAAATACAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTGTATTTATGTTGCTTACTCGAGTAAGCAACATAAATACAGAGG-3'

Other clones with same target sequence:

(none)