Construct: shRNA TRCN0000090865

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023279.2-297s1c1
DNA Barcode:
CAGGCCCGACAACTTTATCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tubb3 (22152)
Gene Description:
tubulin, beta 3 class III
Transcript:
RefSeq NM_023279.2 (CURRENT)
Match location:
Position 297 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090865)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGCCCGACAACTTTATCTT
Hairpin Sequence:
5'-CCGG-CAGGCCCGACAACTTTATCTT-CTCGAG-AAGATAAAGTTGTCGGGCCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGCCCGACAACTTTATCTTCTCGAGAAGATAAAGTTGTCGGGCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGCCCGACAACTTTATCTTCTCGAGAAGATAAAGTTGTCGGGCCTG-3'

Other clones with same target sequence:

(none)