Construct: shRNA TRCN0000094083

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010947.3-1461s1c1
DNA Barcode:
CGGGAGGGCTTCTATCGTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ntn3 (18209)
Gene Description:
netrin 3
Transcript:
RefSeq NM_010947.3 (CURRENT)
Match location:
Position 1461 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094083)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGAGGGCTTCTATCGTGAT
Hairpin Sequence:
5'-CCGG-CGGGAGGGCTTCTATCGTGAT-CTCGAG-ATCACGATAGAAGCCCTCCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGAGGGCTTCTATCGTGATCTCGAGATCACGATAGAAGCCCTCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGAGGGCTTCTATCGTGATCTCGAGATCACGATAGAAGCCCTCCCG-3'

Other clones with same target sequence:

(none)