Construct: shRNA TRCN0000094180

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009961.1-813s1c1
DNA Barcode:
CGAAGGTTCAAATGGTCAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pcdha10 (12943)
Gene Description:
protocadherin alpha 10
Transcript:
RefSeq NM_009961.1 (CURRENT)
Match location:
Position 813 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094180)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAGGTTCAAATGGTCAAAT
Hairpin Sequence:
5'-CCGG-CGAAGGTTCAAATGGTCAAAT-CTCGAG-ATTTGACCATTTGAACCTTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAGGTTCAAATGGTCAAATCTCGAGATTTGACCATTTGAACCTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAGGTTCAAATGGTCAAATCTCGAGATTTGACCATTTGAACCTTCG-3'

Other clones with same target sequence:

(none)