Construct: shRNA TRCN0000094237

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001024478.1-1026s1c1
DNA Barcode:
CCCATCATTTCTCTGGAAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdhr3 (68764)
Gene Description:
cadherin-related family member 3
Transcript:
RefSeq NM_001024478.1 (CURRENT)
Match location:
Position 1026 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094237)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATCATTTCTCTGGAAGTT
Hairpin Sequence:
5'-CCGG-CCCATCATTTCTCTGGAAGTT-CTCGAG-AACTTCCAGAGAAATGATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATCATTTCTCTGGAAGTTCTCGAGAACTTCCAGAGAAATGATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATCATTTCTCTGGAAGTTCTCGAGAACTTCCAGAGAAATGATGGG-3'

Other clones with same target sequence:

(none)