Construct: shRNA TRCN0000094541

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_130457.2-3606s1c1
DNA Barcode:
GCAGTGCAGTTCAGCCATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cntnap4 (170571)
Gene Description:
contactin associated protein-like 4
Transcript:
RefSeq NM_130457.2 (CURRENT)
Match location:
Position 3606 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094541)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTGCAGTTCAGCCATATT
Hairpin Sequence:
5'-CCGG-GCAGTGCAGTTCAGCCATATT-CTCGAG-AATATGGCTGAACTGCACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTGCAGTTCAGCCATATTCTCGAGAATATGGCTGAACTGCACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTGCAGTTCAGCCATATTCTCGAGAATATGGCTGAACTGCACTGC-3'

Other clones with same target sequence:

(none)