Construct: shRNA TRCN0000094919

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033580.1-3591s1c1
DNA Barcode:
GTGGTAAACTAAAGGGAAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pcdhgb8 (93705)
Gene Description:
protocadherin gamma subfamily B, 8
Transcript:
RefSeq NM_033580.1 (NON-CURRENT)
Match location:
Position 3591 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094919)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGTAAACTAAAGGGAAGTT
Hairpin Sequence:
5'-CCGG-GTGGTAAACTAAAGGGAAGTT-CTCGAG-AACTTCCCTTTAGTTTACCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGTAAACTAAAGGGAAGTTCTCGAGAACTTCCCTTTAGTTTACCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGTAAACTAAAGGGAAGTTCTCGAGAACTTCCCTTTAGTTTACCAC-3'

Other clones with same target sequence:

(none)