Construct: shRNA TRCN0000094940

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001003672.1-1811s1c1
DNA Barcode:
CCAAAGTCATAGCCATGGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pcdhac2 (353237)
Gene Description:
protocadherin alpha subfamily C, 2
Transcript:
RefSeq NM_001003672.1 (CURRENT)
Match location:
Position 1811 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094940)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAAGTCATAGCCATGGATT
Hairpin Sequence:
5'-CCGG-CCAAAGTCATAGCCATGGATT-CTCGAG-AATCCATGGCTATGACTTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAAGTCATAGCCATGGATTCTCGAGAATCCATGGCTATGACTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAAGTCATAGCCATGGATTCTCGAGAATCCATGGCTATGACTTTGG-3'

Other clones with same target sequence:

(none)