Construct: shRNA TRCN0000094984

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138663.2-3541s1c1
DNA Barcode:
GCCAGCAGTTCAAGCGTTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pcdha12 (192164)
Gene Description:
protocadherin alpha 12
Transcript:
RefSeq NM_138663.2 (CURRENT)
Match location:
Position 3541 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094984)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGCAGTTCAAGCGTTTAA
Hairpin Sequence:
5'-CCGG-GCCAGCAGTTCAAGCGTTTAA-CTCGAG-TTAAACGCTTGAACTGCTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGCAGTTCAAGCGTTTAACTCGAGTTAAACGCTTGAACTGCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGCAGTTCAAGCGTTTAACTCGAGTTAAACGCTTGAACTGCTGGC-3'

Other clones with same target sequence:

(none)