Construct: shRNA TRCN0000096381

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007488.2-1855s1c1
DNA Barcode:
GAATTGGATCAAGCCACCCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arnt2 (11864)
Gene Description:
aryl hydrocarbon receptor nuclear translocator 2
Transcript:
RefSeq NM_007488.2 (NON-CURRENT)
Match location:
Position 1855 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000096381)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATTGGATCAAGCCACCCTT
Hairpin Sequence:
5'-CCGG-GAATTGGATCAAGCCACCCTT-CTCGAG-AAGGGTGGCTTGATCCAATTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATTGGATCAAGCCACCCTTCTCGAGAAGGGTGGCTTGATCCAATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATTGGATCAAGCCACCCTTCTCGAGAAGGGTGGCTTGATCCAATTC-3'

Other clones with same target sequence:

(none)