Construct: shRNA TRCN0000096912

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133916.1-1814s1c1
DNA Barcode:
CGGGAAGATTGAACTCATCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eif3b (27979)
Gene Description:
eukaryotic translation initiation factor 3, subunit B
Transcript:
RefSeq NM_133916.1 (NON-CURRENT)
Match location:
Position 1814 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000096912)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGAAGATTGAACTCATCAA
Hairpin Sequence:
5'-CCGG-CGGGAAGATTGAACTCATCAA-CTCGAG-TTGATGAGTTCAATCTTCCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGAAGATTGAACTCATCAACTCGAGTTGATGAGTTCAATCTTCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGAAGATTGAACTCATCAACTCGAGTTGATGAGTTCAATCTTCCCG-3'

Other clones with same target sequence:

  1. TRCN0000153861
  2. TRCN0000297919