Construct: shRNA TRCN0000153861

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003751.2-1847s1c1
DNA Barcode:
CGGGAAGATTGAACTCATCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EIF3B (8662)
Gene Description:
eukaryotic translation initiation factor 3 subunit B
Transcript:
RefSeq NM_003751.2 (NON-CURRENT)
Match location:
Position 1847 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000153861)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGAAGATTGAACTCATCAA
Hairpin Sequence:
5'-CCGG-CGGGAAGATTGAACTCATCAA-CTCGAG-TTGATGAGTTCAATCTTCCCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGAAGATTGAACTCATCAACTCGAGTTGATGAGTTCAATCTTCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGAAGATTGAACTCATCAACTCGAGTTGATGAGTTCAATCTTCCCG-3'

Other clones with same target sequence:

  1. TRCN0000096912
  2. TRCN0000297919