Construct: shRNA TRCN0000098769

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172124.2-1480s1c1
DNA Barcode:
CAATCCGAAAGCTGTATTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
B3gat2 (280645)
Gene Description:
beta-1,3-glucuronyltransferase 2 (glucuronosyltransferase S)
Transcript:
RefSeq NM_172124.2 (CURRENT)
Match location:
Position 1480 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000098769)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAATCCGAAAGCTGTATTTAA
Hairpin Sequence:
5'-CCGG-CAATCCGAAAGCTGTATTTAA-CTCGAG-TTAAATACAGCTTTCGGATTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAATCCGAAAGCTGTATTTAACTCGAGTTAAATACAGCTTTCGGATTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAATCCGAAAGCTGTATTTAACTCGAGTTAAATACAGCTTTCGGATTG-3'

Other clones with same target sequence:

(none)