Construct: shRNA TRCN0000098962

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015795.1-389s1c1
DNA Barcode:
CCAAGCTTCCAAGGGTGTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fbxo16 (50759)
Gene Description:
F-box protein 16
Transcript:
RefSeq NM_015795.1 (CURRENT)
Match location:
Position 389 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000098962)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGCTTCCAAGGGTGTTAT
Hairpin Sequence:
5'-CCGG-CCAAGCTTCCAAGGGTGTTAT-CTCGAG-ATAACACCCTTGGAAGCTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGCTTCCAAGGGTGTTATCTCGAGATAACACCCTTGGAAGCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGCTTCCAAGGGTGTTATCTCGAGATAACACCCTTGGAAGCTTGG-3'

Other clones with same target sequence:

(none)