Construct: shRNA TRCN0000099950

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027375.2-5487s1c1
DNA Barcode:
GCCGTGTTTATTAAAGGATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gcc2 (70297)
Gene Description:
GRIP and coiled-coil domain containing 2
Transcript:
RefSeq NM_027375.2 (CURRENT)
Match location:
Position 5487 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000099950)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGTGTTTATTAAAGGATTA
Hairpin Sequence:
5'-CCGG-GCCGTGTTTATTAAAGGATTA-CTCGAG-TAATCCTTTAATAAACACGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGTGTTTATTAAAGGATTACTCGAGTAATCCTTTAATAAACACGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGTGTTTATTAAAGGATTACTCGAGTAATCCTTTAATAAACACGGC-3'

Other clones with same target sequence:

(none)