Construct: shRNA TRCN0000100222

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_130455.2-1651s1c1
DNA Barcode:
CCTTTGACTTTGAGCTCTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Grin3b (170483)
Gene Description:
glutamate receptor, ionotropic, NMDA3B
Transcript:
RefSeq NM_130455.2 (CURRENT)
Match location:
Position 1651 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100222)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTGACTTTGAGCTCTATA
Hairpin Sequence:
5'-CCGG-CCTTTGACTTTGAGCTCTATA-CTCGAG-TATAGAGCTCAAAGTCAAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTGACTTTGAGCTCTATACTCGAGTATAGAGCTCAAAGTCAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTGACTTTGAGCTCTATACTCGAGTATAGAGCTCAAAGTCAAAGG-3'

Other clones with same target sequence:

(none)