Construct: shRNA TRCN0000100311

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028809.1-479s1c1
DNA Barcode:
GCTCCATTATAAGAGTTCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arpc5l (74192)
Gene Description:
actin related protein 2/3 complex, subunit 5-like
Transcript:
RefSeq NM_028809.1 (CURRENT)
Match location:
Position 479 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100311)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCCATTATAAGAGTTCTTA
Hairpin Sequence:
5'-CCGG-GCTCCATTATAAGAGTTCTTA-CTCGAG-TAAGAACTCTTATAATGGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCCATTATAAGAGTTCTTACTCGAGTAAGAACTCTTATAATGGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCCATTATAAGAGTTCTTACTCGAGTAAGAACTCTTATAATGGAGC-3'

Other clones with same target sequence:

(none)