Construct: shRNA TRCN0000100511

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027135.2-2419s1c1
DNA Barcode:
GCTGTGAAACTGATGCCCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sec24d (69608)
Gene Description:
Sec24 related gene family, member D (S. cerevisiae)
Transcript:
RefSeq NM_027135.2 (CURRENT)
Match location:
Position 2419 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100511)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTGAAACTGATGCCCTTA
Hairpin Sequence:
5'-CCGG-GCTGTGAAACTGATGCCCTTA-CTCGAG-TAAGGGCATCAGTTTCACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTGAAACTGATGCCCTTACTCGAGTAAGGGCATCAGTTTCACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTGAAACTGATGCCCTTACTCGAGTAAGGGCATCAGTTTCACAGC-3'

Other clones with same target sequence:

(none)