Construct: shRNA TRCN0000100513

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027135.2-1128s1c1
DNA Barcode:
CCCTTGTACTTGGTAAACCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sec24d (69608)
Gene Description:
Sec24 related gene family, member D (S. cerevisiae)
Transcript:
RefSeq NM_027135.2 (CURRENT)
Match location:
Position 1128 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100513)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTGTACTTGGTAAACCAT
Hairpin Sequence:
5'-CCGG-CCCTTGTACTTGGTAAACCAT-CTCGAG-ATGGTTTACCAAGTACAAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTGTACTTGGTAAACCATCTCGAGATGGTTTACCAAGTACAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTGTACTTGGTAAACCATCTCGAGATGGTTTACCAAGTACAAGGG-3'

Other clones with same target sequence:

(none)