Construct: shRNA TRCN0000100545

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181072.3-4603s1c1
DNA Barcode:
CCTCCCTTACTTCCTAAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Myo1e (71602)
Gene Description:
myosin IE
Transcript:
RefSeq NM_181072.3 (CURRENT)
Match location:
Position 4603 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100545)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCCTTACTTCCTAAATAA
Hairpin Sequence:
5'-CCGG-CCTCCCTTACTTCCTAAATAA-CTCGAG-TTATTTAGGAAGTAAGGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCCTTACTTCCTAAATAACTCGAGTTATTTAGGAAGTAAGGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCCTTACTTCCTAAATAACTCGAGTTATTTAGGAAGTAAGGGAGG-3'

Other clones with same target sequence:

  1. TRCN0000288008