Construct: shRNA TRCN0000102445

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021525.2-1350s1c1
DNA Barcode:
CCTCTTAACATTACAGACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rcl1 (59028)
Gene Description:
RNA terminal phosphate cyclase-like 1
Transcript:
RefSeq NM_021525.2 (CURRENT)
Match location:
Position 1350 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102445)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTTAACATTACAGACTTT
Hairpin Sequence:
5'-CCGG-CCTCTTAACATTACAGACTTT-CTCGAG-AAAGTCTGTAATGTTAAGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTTAACATTACAGACTTTCTCGAGAAAGTCTGTAATGTTAAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTTAACATTACAGACTTTCTCGAGAAAGTCTGTAATGTTAAGAGG-3'

Other clones with same target sequence:

(none)