Construct: shRNA TRCN0000102909

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_134160.1-1260s1c1
DNA Barcode:
GCTATGATCTATCTAGGCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mcoln3 (171166)
Gene Description:
mucolipin 3
Transcript:
RefSeq NM_134160.1 (CURRENT)
Match location:
Position 1260 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102909)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATGATCTATCTAGGCTAT
Hairpin Sequence:
5'-CCGG-GCTATGATCTATCTAGGCTAT-CTCGAG-ATAGCCTAGATAGATCATAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATGATCTATCTAGGCTATCTCGAGATAGCCTAGATAGATCATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATGATCTATCTAGGCTATCTCGAGATAGCCTAGATAGATCATAGC-3'

Other clones with same target sequence:

(none)