Construct: shRNA TRCN0000103045

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008165.2-3444s1c1
DNA Barcode:
CCCAGAAAGAATACTACCTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gria1 (14799)
Gene Description:
glutamate receptor, ionotropic, AMPA1 (alpha 1)
Transcript:
RefSeq NM_008165.2 (NON-CURRENT)
Match location:
Position 3444 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103045)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGAAAGAATACTACCTAA
Hairpin Sequence:
5'-CCGG-CCCAGAAAGAATACTACCTAA-CTCGAG-TTAGGTAGTATTCTTTCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGAAAGAATACTACCTAACTCGAGTTAGGTAGTATTCTTTCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGAAAGAATACTACCTAACTCGAGTTAGGTAGTATTCTTTCTGGG-3'

Other clones with same target sequence:

(none)