Construct: shRNA TRCN0000103110

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145142.2-1823s1c1
DNA Barcode:
CCCAGCACTTTCAAGAGAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Chst10 (98388)
Gene Description:
carbohydrate sulfotransferase 10
Transcript:
RefSeq NM_145142.2 (CURRENT)
Match location:
Position 1823 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103110)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGCACTTTCAAGAGAGAT
Hairpin Sequence:
5'-CCGG-CCCAGCACTTTCAAGAGAGAT-CTCGAG-ATCTCTCTTGAAAGTGCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGCACTTTCAAGAGAGATCTCGAGATCTCTCTTGAAAGTGCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGCACTTTCAAGAGAGATCTCGAGATCTCTCTTGAAAGTGCTGGG-3'

Other clones with same target sequence:

(none)