Transcript: Mouse NM_145142.2

Mus musculus carbohydrate sulfotransferase 10 (Chst10), mRNA.

Source:
NCBI, updated 2017-05-14
Taxon:
Mus musculus (mouse)
Gene:
Chst10 (98388)
Length:
3135
CDS:
370..1494

Additional Resources:

NCBI RefSeq record:
NM_145142.2
NBCI Gene record:
Chst10 (98388)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_145142.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000103111 GCATAGACCATCTGGTGTCAT pLKO.1 1316 CDS 100% 4.950 6.930 N Chst10 n/a
2 TRCN0000103113 GAAACCAGATTTCTTGCTAAA pLKO.1 1470 CDS 100% 10.800 7.560 N Chst10 n/a
3 TRCN0000103114 GTCTGTGACAAGCACAAGATT pLKO.1 766 CDS 100% 5.625 3.938 N Chst10 n/a
4 TRCN0000103110 CCCAGCACTTTCAAGAGAGAT pLKO.1 1823 3UTR 100% 4.950 3.465 N Chst10 n/a
5 TRCN0000103112 CCTTGGTACAGGCATGAGATA pLKO.1 1054 CDS 100% 4.950 3.465 N Chst10 n/a
6 TRCN0000034761 CAGTGGAAGAAAGTGCTGATT pLKO.1 820 CDS 100% 4.950 3.465 N CHST10 n/a
7 TRCN0000034760 CCTTGGTACAGGCATGAGATT pLKO.1 1054 CDS 100% 4.950 3.465 N CHST10 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_145142.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07426 pDONR223 100% 82.4% 86.6% None (many diffs) n/a
2 ccsbBroad304_07426 pLX_304 0% 82.4% 86.6% V5 (many diffs) n/a
3 TRCN0000475427 TGTTCGCAGTTGGTGGCCTTGTTC pLX_317 38.6% 82.4% 86.6% V5 (many diffs) n/a
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