Construct: shRNA TRCN0000103230

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013541.1-352s1c1
DNA Barcode:
CGGCAAATATGTCACCCTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gstp1 (14870)
Gene Description:
glutathione S-transferase, pi 1
Transcript:
RefSeq NM_013541.1 (CURRENT)
Match location:
Position 352 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103230)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCAAATATGTCACCCTCAT
Hairpin Sequence:
5'-CCGG-CGGCAAATATGTCACCCTCAT-CTCGAG-ATGAGGGTGACATATTTGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCAAATATGTCACCCTCATCTCGAGATGAGGGTGACATATTTGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCAAATATGTCACCCTCATCTCGAGATGAGGGTGACATATTTGCCG-3'

Other clones with same target sequence:

  1. TRCN0000353988