Construct: shRNA TRCN0000105476

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008648.1-125s1c1
DNA Barcode:
CTTCCAAGGGACAGAACTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mup4 (17843)
Gene Description:
major urinary protein 4
Transcript:
RefSeq NM_008648.1 (CURRENT)
Match location:
Position 125 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000105476)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCCAAGGGACAGAACTTAA
Hairpin Sequence:
5'-CCGG-CTTCCAAGGGACAGAACTTAA-CTCGAG-TTAAGTTCTGTCCCTTGGAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCCAAGGGACAGAACTTAACTCGAGTTAAGTTCTGTCCCTTGGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCCAAGGGACAGAACTTAACTCGAGTTAAGTTCTGTCCCTTGGAAG-3'

Other clones with same target sequence:

(none)