Construct: shRNA TRCN0000105627

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019575.4-716s1c1
DNA Barcode:
GCCCAGTTTAACAGTTTCTCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Scamp4 (56214)
Gene Description:
secretory carrier membrane protein 4
Transcript:
RefSeq NM_019575.4 (CURRENT)
Match location:
Position 716 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000105627)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAGTTTAACAGTTTCTCT
Hairpin Sequence:
5'-CCGG-GCCCAGTTTAACAGTTTCTCT-CTCGAG-AGAGAAACTGTTAAACTGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAGTTTAACAGTTTCTCTCTCGAGAGAGAAACTGTTAAACTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAGTTTAACAGTTTCTCTCTCGAGAGAGAAACTGTTAAACTGGGC-3'

Other clones with same target sequence:

  1. TRCN0000324177