Construct: shRNA TRCN0000105648

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020506.1-1159s1c1
DNA Barcode:
GCGTTGGAAGAAGTGCTTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Xpo4 (57258)
Gene Description:
exportin 4
Transcript:
RefSeq NM_020506.1 (CURRENT)
Match location:
Position 1159 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000105648)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTTGGAAGAAGTGCTTGAT
Hairpin Sequence:
5'-CCGG-GCGTTGGAAGAAGTGCTTGAT-CTCGAG-ATCAAGCACTTCTTCCAACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTTGGAAGAAGTGCTTGATCTCGAGATCAAGCACTTCTTCCAACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTTGGAAGAAGTGCTTGATCTCGAGATCAAGCACTTCTTCCAACGC-3'

Other clones with same target sequence:

  1. TRCN0000324183