Construct: shRNA TRCN0000105914

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172803.2-3932s1c1
DNA Barcode:
CCGCTACATCCACAAACTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dock4 (238130)
Gene Description:
dedicator of cytokinesis 4
Transcript:
RefSeq NM_172803.2 (CURRENT)
Match location:
Position 3932 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000105914)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCTACATCCACAAACTCTA
Hairpin Sequence:
5'-CCGG-CCGCTACATCCACAAACTCTA-CTCGAG-TAGAGTTTGTGGATGTAGCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCTACATCCACAAACTCTACTCGAGTAGAGTTTGTGGATGTAGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCTACATCCACAAACTCTACTCGAGTAGAGTTTGTGGATGTAGCGG-3'

Other clones with same target sequence:

(none)