Construct: shRNA TRCN0000105934

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177364.3-486s1c1
DNA Barcode:
GAGCATATTGGGAAGAAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sh3pxd2b (268396)
Gene Description:
SH3 and PX domains 2B
Transcript:
RefSeq NM_177364.3 (CURRENT)
Match location:
Position 486 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000105934)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCATATTGGGAAGAAGAAA
Hairpin Sequence:
5'-CCGG-GAGCATATTGGGAAGAAGAAA-CTCGAG-TTTCTTCTTCCCAATATGCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCATATTGGGAAGAAGAAACTCGAGTTTCTTCTTCCCAATATGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCATATTGGGAAGAAGAAACTCGAGTTTCTTCTTCCCAATATGCTC-3'

Other clones with same target sequence:

(none)